CD4+CD25+调节性T细胞回输对大鼠肝移植急性排斥反
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浦立勇, 李相成, 王学浩, 张 峰, 姚爱华,
细胞磁分离技术;CD4+CD25+调节性T细胞;大鼠;肝移植;急性排斥反应浦立勇,李相成,王学浩,张峰,姚爱华,俞悦,吕凌,李国强.CD4+CD25+调节性T细胞回输对大鼠肝移植急性排斥反应的影响.世
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浦立勇, 李相成, 王学浩, 张峰, 姚爱华, 俞悦, 吕凌, 李国强, 南京医科大学第一附属医院肝脏移植中心 江苏省南京市 210029
浦立勇, 男, 1973-03-22生, 江苏省盐城市人, 汉族, 博士研究生, 主治医师, 主要从事肝脏外科与肝脏移植免疫学研究.
国家自然科学基金资助项目, No. 30271236
通讯作者: 王学浩, 210029, 江苏省南京市广州路300号, 南京医科大学第一附属医院肝脏移植中心. wangxh@njmu.edu.cn
电话: 025-83718836-6476 传真:025-83724440
收稿日期: 2005-12-08 接受日期:2005-12-31
Effect of infusing CD4+CD25+ regulatory T cell on the rejection model of DA-Lewis rat liver transplantation
Li-Yong Pu, Xiang-Cheng Li, Xue-Hao Wang, Feng Zhang, Ai-Hua Yao, Yue Yu, Ling Lv, Guo-Qiang Li
Li-Yong Pu, Xiang-Cheng Li, Xue-Hao Wang, Feng Zhang, Ai-Hua Yao, Yue Yu, Ling Lv, Guo-Qiang Li, Center of Liver Transplantation, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Supported by National Natural Science Foundation of China, No.30271236
correspondence to: Dr. Xue-Hao Wang, Center of Liver Transplantation, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. wangxh@njmu.edu.cn
Received: 2005-12-08 Accepted:2005-12-31
Abstract
AIM: To investigate the effect of infusing ex vivo isolated CD4+CD25+ regulatory T cells on the rejection model of DA-Lewis rat liver transplantation.
METHODS:CD4+CD25+ regulatory T cells were isolated from Lewis (RT11) rat splenocytes and peripheral lymph nodes by magnetic cell sorting system (negative selection and positive selection), and co-cultured with mitomycin-C (MMC) treated dark agouti (RT1a, DA) rat splenocytes for 3 d. The suppressive efficiency of fresh or ex vivo stimulated CD4+CD25+ regulatory T cells on the proliferation of CD4+CD25- T cells was evaluated by cell proliferation assay. Four groups (12 rats for each) of orthotopic liver transplants (OLT, DA into Lewis) were established by modified Kamada two-cuff technique ......
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