【摘要】 目的 从分子水平分析四川省不同地区、不同年代O1群霍乱弧菌的致病性以及菌株分子特征和相互关系；为了解四川省霍乱流行规律、预测疫情、制定防治措施提供理论依据。方法 运用打点杂交或PCR方法检测霍乱弧菌毒力基因ctxA，16S rRNA探针Southern杂交进行核糖体基因分型分析等。结果 所试菌株2株ctxA基因阴性，其余阳性；16S rRNA核糖体分型可分为6个RT型。结论 除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以前四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起大流行的稻叶1d为同一RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

    【关键词】 霍乱弧菌；O1群；分子流行病学；特征

    The molecular epidemiology character of Vibrio cholerae O1 in Sichuan province

    Liu Hong-lu，Luo Long-ze，Feng Ze-hui, et al.

    Center for Disease Control and Prevention of Sichuan Province, Chengdu 610031, China

    【Abstract】 Objective To analyse pathogenicity of Vibrio cholerae O1 from different areas and different years, to understand the molecular characters and relations between strains, on the purpose of providing a basis for epidemic prediction and policy making for cholera control.Methods Testing toxic gene ctxA by Dot hybridization, ribotyping by 16S rRNA probe Southern hybridization. Results All strains were ctxA positive except two. 16S rRNA typed 6 patterns. Conclusion All strains were pathogenetic except 82-007 and 95-001. Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone. ......