【摘要】 目的 建立一种快速检测抗H.pylori IgG抗体的胶体金免疫层析试纸条，并优化制备中各关键步骤的实验条件。方法 以柠檬酸三钠还原法制备20nm的胶体金溶液，葡萄球菌A蛋白(SPA)，制备免疫胶体金复合物，组装胶体金免疫层析快速诊断试纸条。结果 用柠檬酸还原法制备的20nm胶体金溶液呈亮红色。胶体金标记SPA的最低稳定浓度5μg/ml，最适稳定量为6μg/ ml。SPA标记的最适pH为6.0。以此试纸条检测临床血清标本与进口ELISA试剂盒比较，组间阳性率比较差异无显著性。结论 胶体金免疫层析试纸条质量稳定性不但与抗原抗体的选择、用量优化、层析材料的选择、胶体金的制备与标记等因素密切相关，而且缓冲系统的优化、辅助添加剂的选择与优化组合也非常重要。

    【关键词】 幽门螺杆菌；胶体金；免疫层析检测

    Research on development of gold immunochromatographic test kit for serum H.pylori antibody IgG

    ZHANG Bao-yuan，WANG Xiao-wei，CUI Xiao-dai，et al.Capital Institute of Pediatrics，Beijing 100020，China

    【Abstract】 Objective Developing gold immunochromatographic test kit for serum H.pylori antibody IgG and optimize the key experiment conditions.Methods Preparing the colloid gold particles of 20 nm which were coupled with Streptococcus protein A (SPA)，set up the gold immunochromatography test kit for serum H.pylori antibody IgG.Results The spheroidal colloid gold particles of 20nm is shown in color of bright red.The minimal stable concentration (MSC) of gold- SPA is 5μg/ml，and the suitable stable concentration ( SSC) is 6μg/ml.The pH 6.0 is appropriate. 53 sera samples were tested by GICA and ELISA ......