Treatment of cerebral hemorrhage with stem cell tr
Research fellows of the laboratory of cytology, Beijing Institute of Neurosurgery, Beijing Tiantan Hospital announced one of the experimental investigations showed that intra-cerebral transplantation of neuro-stem cells close to the site of injury might effectively improve the motor functions in animal models of cerebral hemorrhage. The researchers isolated nerve stem cells from the cerebral tissue of rat embryo of 16th days of gestational age, cultured them and induced differentiation of the cells and their special features were studied with immunohistochemical technique. They randomly allocated 41 male rats into four groups: 1. transplantation of neuro-stem cells in rats without hematoma; (chiefly for the detection of the influence to the motor function of the rats following neuro-stem cell transplantation); 2. control group with hematoma; 3. hematoma with ipsilateral neuro-stem cell transplantation and hematoma with contralateral transplantation of neuro-stem cell. The purpose of this investigation was to observe the feasibility of cerebral hemorrhage treatment with the transplantation of rat embryo neuro-stem cells.
The results of the study showed that the stem cells had a strong proliferating ability and a high potential of multi-differentiation. When these cells were transplanted into the contra-lateral side of the injury, they migrated into the side of injury, and in a period of 6 weeks, they formed a cohort of a thin tip and a broad tail along the callosal gyrus and the corpus callosum. The linear distance from the tip of the cohort to the nearest point of injection was more than 3 mm, indicating a very strong power of migration. When the transplantation was performed at the side of hematoma, a large number of neuro-stem cells differentiated into the astrocytes, encircling the area with the hemorrhage focus as the center, and only a small number of them differentiated into neurons and the oligodendrocytes, indicating a trend of oriented differentiation of the transplanted stem cells according to the external circumstances., http://www.100md.com
The results of the study showed that the stem cells had a strong proliferating ability and a high potential of multi-differentiation. When these cells were transplanted into the contra-lateral side of the injury, they migrated into the side of injury, and in a period of 6 weeks, they formed a cohort of a thin tip and a broad tail along the callosal gyrus and the corpus callosum. The linear distance from the tip of the cohort to the nearest point of injection was more than 3 mm, indicating a very strong power of migration. When the transplantation was performed at the side of hematoma, a large number of neuro-stem cells differentiated into the astrocytes, encircling the area with the hemorrhage focus as the center, and only a small number of them differentiated into neurons and the oligodendrocytes, indicating a trend of oriented differentiation of the transplanted stem cells according to the external circumstances., http://www.100md.com