胃电刺激对大鼠胃扩张反应神经元及增食欲素的影响
http://www.100md.com
孙向荣, 徐 珞, 叶俊丽
胃电刺激;下丘脑腹内侧核;增食欲素;免疫荧光组织化学;放射免疫;大鼠,孙向荣,徐珞,叶俊丽,孙向荣,通讯作者:,Effectsofgastricel
 |
| 第2页 |
 |
| 第1页 |
参见附件(554KB，6页)。
孙向荣, 徐珞, 叶俊丽, 青岛大学医学院病理生理教研室 山东省青岛市 266021
孙向荣, 女, 1977-03-05生, 山西省宁武县人, 汉族, 2002年青岛大学医学院硕士, 讲师, 主要从事神经内分泌研究.
国家自然科学基金资助项目, No.30470642
青岛市科技局资助项目, No.05-1-JC-93
通讯作者: 徐珞, 266021, 青岛市登州路38号, 青岛大学医学院病理生理教研室. luoxukai@yahoo.com
电话: 0532-83056510 传真: 0532-83813986
收稿日期: 2005-11-30 接受日期: 2005-12-06
Effects of gastric electrical stimulation on responsive neurons to gastric distension and expression of orexin in rats
Xiang-Rong Sun, Luo Xu, Jun-Li Ye
Xiang-Rong Sun, Luo Xu, Jun-Li Ye, Department of Pathophysiology, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Supported by the National Natural Science Foundation of China, No. 30470642, and Qingdao Scientific and Technique Division, No. 05-1-JC-93
Correspondence to: Dr. Luo Xu, Department of Pathophysiology, Medical College of Qingdao University, 38 Dengzhou Road, Qingdao 266021, Shandong Province, China. luoxukai@yahoo.com
Received: 2005-11-30 Accepted: 2005-12-06
Abstract
AIM: To investigate the effects of gastric electri-cal stimulation (GES) on responsive neurons to gastric distension (GD) in ventromedia hypotha-lamus (VMH) and the expression of orexin in rat brain.
METHODS: Fifty-two adult Wistar rats were used in this experiment. The effects of GES on GD responsive neurons in VMH were observed by recording extracellular potentials of single neuron. GD responsive neurons were classified as GD-excitatory (GD-E) and GD-inhibitory (GD-I) ones according to their responses to GD. GES with three sets of parameters were applied for one minute respectively: GES1 (6 mA, 0.3 ms, 40 Hz, 2 s-on, 3 s-off) with standard pulse trains; GES2 with reduced on-time to 0.1 s and GES3 with decreased frequency to 20 Hz. After GES1 was using for 2 h, we observed the expression of orexin-A immunoreactive (orexin-A-IR) positive neurons in lateral hypothalamus area (LHA) by fluorescent immunohistochemistry and the content of orexin in rat brain by radioimmunoassay ......
您现在查看是摘要介绍页，详见PDF附件(554KB，6页)。