趋化性细胞因子MIP-1a动员DC前体细胞的体内抗胃癌效应
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何宋兵, 汪 良, 黄 瑞, 张雁云
树突状细胞;巨噬细胞炎症蛋白-1a;抗肿瘤;胃癌,何宋兵,汪良,何宋兵,张雁云,黄瑞,何宋兵,通讯作者:,Anti-gastricc
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何宋兵, 汪良, 苏州大学附属第一医院普外科 江苏省苏州市 215006
何宋兵, 张雁云, 上海交通大学医学院上海市免疫学研究所 上海市 200025
黄瑞, 苏州大学医学院微生物学教研室 江苏省苏州市 215006
何宋兵, 男, 1979-12-19 生, 江苏省昆山市人, 汉族, 苏州大学附属第一医院普外科, 硕士研究生, 主要从事消化道肿瘤的基础和临床研究.
通讯作者: 张雁云, 200025, 上海市重庆南路227号, 上海交通大学医学院上海市免疫学研究所. yyzhang@sibs.ac.cn
电话: 021-63852705 传真: 021-63852705
收稿日期: 2005-11-30 接受日期: 2005-12-19
Anti-gastric carcinoma efficacy of macrophage inflammation protein-1a recruited dendritic cell vaccine in mice
Song-Bing He, Liang Wang, Rui Huang, Yan-Yun Zhang
Song-Bing He, Liang Wang, Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
Song-Bing He, Yan-Yun Zhang, Shanghai Institute of Immunology, Medical College of Shanghai Jiao Tong University, Shanghai 200025, China
Rui Huang, Department of Microbiology, Medical College of Soochow University, Suzhou 215006, Jiangsu Province, China
Correspondence to: Dr. Yan-Yun Zhang, Shanghai Institute of Immunology, Medical College of Shanghai Jiao Tong University, 227 South Chongqing Road, Shanghai 200025, China. yyzhang@sibs.ac.cn
Received: 2005-11-30 Accepted: 2005-12-19
Abstract
AIM: To study the in vivo anti-tumor effects of dendritic cell (DC) precursors after being recruited in the peripheral blood by injection of macrophage inflammation protein-1a (MIP-1a) and pulsed by the mouse forestomach carcinoma cell (MFC) lysates.
METHODS: B220-CD11c+ cells were sorted from the peripheral blood of C57BL/6 mice after injection of MIP-1a via tail vein, and then cultured for 5-6 d in the presence of granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and mouse tumor necrosis factor (mTNF-a). The phenotypes and mixed lymphocyte reaction (MLR) of these cells were assayed. DC vaccines were prepared after B220-CD11c+ cells were loaded with MFC tumor antigen, and then were transfused in the mice baring MFC ......
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