重症急性胰腺炎大鼠肺组织中 LIF 的表达变化及意义
http://www.100md.com
滕晓丽, 赵 秋, 杜 静, 谷 华, 覃 华
重症急性胰腺炎;白血病抑制因子;肺损伤,重症急性胰腺炎大鼠肺组织中LIF的表达变化及意义,滕晓丽,赵秋,杜静,谷华,覃华,通讯作者:,Roleofleukemiainhibitoryfactoron
 |
| 第1页 |
参见附件(873KB，4页)。
滕晓丽, 赵秋, 杜静, 谷华, 覃华, 华中科技大学同济医学院附属同济医院消化内科 湖北省武汉市 430030
通讯作者: 赵秋, 430030, 湖北省武汉市, 华中科技大学同济医学院附属同济医院消化内科. dalianmao2121@hotmail.com
电话: 027-83663611
收稿日期: 2005-12-01接受日期: 2005-12-19
Role of leukemia inhibitory factor on lung injury in rats with severe acute pancreatitis
Xiao-Li Teng, Qiu Zhao, Jing Du, Hua Gu, Hua Qin
Xiao-Li Teng, Qiu Zhao, Jing Du, Hua Gu, Hua Qin, Department of Gastroenterology, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to: Qiu Zhao, Department of Gastroenterology, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province,China. dalianmao2121@hotmail.com
Received: 2005-12-01 Accepted: 2005-12-19
Abstract
AIM: To observe the levels of leukemia inhibitory factor (LIF) in the pulmonary tissues in rats with severe acute pancreatitis (SAP), and to investigate the role of LIF in the progression of SAP and lung injury.
METHODS: Thirty-six male Sprague Dawley rats were randomly divided into three groups: normal control (n = 6), sham operation (n = 6), and SAP (n = 24) group. SAP model was induced by retrograde injection of 50 g/L sodium taurocholate into the pancreatobiliary duct. The expression of pulmonary LIF mRNA was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and the expression of LIF protein was detected by immunohistochemistry.
RESULTS: The level of pulmonary LIF mRNA expression was increased significantly in SAP group at 3 h in comparison with that in normal control and sham operation group (grey value: 1.018 ± 0.065 vs 1.451 ± 0.067, 1.322 ± 0.072, bothP < 0.05), and maintained at high levels up to 24 h after modeling (0.853 ± 0.058, 0.635 ± 0.064, 0.582 ± 0 ......
您现在查看是摘要介绍页，详见PDF附件(873KB，4页)。