应用 MS-RDA 筛选胃癌甲基化差异片段
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廖爱军, 苏 琦, 王 珣, 曾 斌, 石 巍
胃癌;DNA甲基化;差异片段;MS-RDA,应用MS-RDA筛选胃癌甲基化差异片段,廖爱军,苏琦,王珣,曾斌,石巍,通讯作者:,Isolationofdifferentiallymethylat
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廖爱军, 南华大学肿瘤研究所, 南华大学附属第一医院消化内科 湖南省衡阳市 421001
苏琦, 南华大学肿瘤研究所 湖南省衡阳市 421001
王珣, 曾斌, 石巍, 南华大学附属第一医院消化内科 湖南省衡阳市 421001
廖爱军, 女, 1964-08-08生, 湖南省宁乡县人, 汉族, 2004年南华大学肿瘤研究所病理学博士生, 教授, 主要从事胃癌病因、发病机制及防治的研究.
湖南省科研计划重大专项, No 04SK1004
湖南省教育厅科学研究资助项目, No.99C195
通讯作者: 苏琦, 421001, 湖南省衡阳市, 南华大学肿瘤研究所. suqi1@hotmail.com
电话: 0734-8279064 传真: 0734-8281547
收稿日期: 2005-11-23 接受日期: 2005-11-25
Isolation of differentially methylated DNA sequences of gastric cancer by methylation-sensitive representational difference analysis
Ai-Jun Liao, Qi Su, Xun Wang, Bin Zeng, Wei Shi
Ai-Jun Liao, Institute of Cancer Research, Nanhua University; Department of Gastroenterology, the First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China
Qi Su, Institute of Cancer Research, Nanhua University, Hengyang 421001, Hunan Province, China
Xun Wang, Bin Zeng, Wei Shi, Department of Gastroenterology, the First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China
Supported by the Key Foundation of Science and Technology Program of Hunan Province, No.04SK1004, and the Scientific Research Foundation from Education Department of Hunan Province, No. 99C195
Correspondence to: Dr. Qi Su, Cancer Research Institute, Nanhua University, Hengyang 421001, Hunan Province China. suqi1@hotmail.com
Received: 2005-11-23 Accepted: 2005-11-25
Abstract
AIM: To isolate the differentially methylated DNA sequences between gastric cancer and normal gastric mucosa.
METHODS: The differentially methylated DNA sequences between gasrtic cancer and normal gastric mucosa were isolated by methylation-sensitive presentational difference analysis (MS-RDA). Similarities between the separated fragments and the human genomic DNA were analyzed with Building Loads Analysis and System Thermodynamics (BLAST) program ......
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