靶向siRNA抑制幽门螺杆菌vacA表达
http://www.100md.com
赵 刚, 詹文华, 严燕国, 马晋平, 彭俊生,
RNA干扰;空泡毒素A;幽门螺杆菌,赵刚,詹文华,严燕国,马晋平,彭俊生,董文广,蔡世荣,何裕隆,通讯作者:,GangZhao,Wen-HuaZhan
 |
| 第5页 |
 |
| 第1页 |
参见附件(989KB，6页)。
赵刚, 詹文华, 严燕国, 马晋平, 彭俊生, 董文广, 蔡世荣, 何裕隆, 中山大学附属第一医院胃肠胰外科, 中山大学胃癌诊治中心 广东省广州市 510080赵刚, 男, 1968-03-07生, 哈尔滨人, 汉族, 2001年哈尔滨医科大学医学博士, 2003年中山大学附属第一医院胃肠胰外科博士后, 副教授, 副主任医师, 主要从事胃肠道肿瘤外科和分子生物治疗方面的研究.
国家自然基金资助项目, No. 30271276
广东省自然科学基金, No. 021891
通讯作者: 詹文华, 510080, 广东省广州市, 中山大学附属第一医院胃肠胰外科, 中山大学胃癌诊治中心. wenhuazh@21cn.com电话: 020-88533958
收稿日期: 2005-11-08 接受日期: 2005-12-10
Small interfering RNA targeted inhibition of vacA expression in Helicobacter pylori
Gang Zhao, Wen-Hua Zhan, Yan-Guo Yan, Jin-Ping Ma, Jun-Sheng Peng, Wen-Guang Dong, Shi-Rong Cai,Yu-Long He
Gang Zhao, Wen-Hua Zhan, Yan-Guo Yan, Jin-Ping Ma, Jun-Sheng Peng, Wen-Guang Dong, Shi-Rong Cai, Yu-Long He, Department of Gastrointestinal Surgery, the First Affiliated Hospital Sun Yat-sen University; Gastric Cancer Center of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
Supported by National Natural Science Foundation of China, No. 30271276, and the Natural Science Foundation of Guangdong Province, No. 021891
Correspondence to: Dr. Wen-Hua Zhan, Department of Gastrointestinal Surgery, the First Affiliated Hospital Sun Yat-sen University; Gastric Cancer Center of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China. wenhuazh@21cn.com
Received: 2005-11-08 Accepted: 2005-12-10
Abstract
AIM: To investigate whether RNA interference (RNAi) induced by small interfering RNA (siRNA) can suppress the expression of vacA in Helicobacter pylori.
METHODS: Five pairs of siRNA targeted vacA, named vacA-s1, vacA-s2, vacA-s3, vacA-s4, and vacA-s5, and one pair non-specific siRNA were synthesized and then transferred into H pylori using electroporation method. Before and 1, 6, 12, 24, and 48 h after electroporation, the expression of vacA gene and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot, respectively. PCR products were also cloned and sequenced ......
您现在查看是摘要介绍页，详见PDF附件(989KB，6页)。