复方肝癌宁含药血清诱导肝癌HepG2细胞调亡的实验研究
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袁冬生, 王新华, 李常青, 刘妮
复方肝癌宁;HepG2;流式细胞术;细胞凋亡;血清药理学袁冬生,王新华,李常青,刘妮.复方肝癌宁含药血清诱导肝癌HepG2细胞调亡的实验研究.世界华人消化杂志2006;14(5)522-525http
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袁冬生,广州中医药大学职业技术学院 广东省广州市 510405
王新华,广州中医药大学校长办公室 广东省广州市 510405
李常青,刘妮, 广州中医药大学热带医学研究所 广东省广州市 510405
袁冬生, 男, 1964-10-02生,江西乐安县人,汉族, 博士研究生, 研究员, 硕士生导师. 主要研究方向为中医药治疗乙型肝炎的基础研究.
广东省科技厅科技计划资助项目, No.2005B33001032
通讯作者: 袁冬生, 510405, 广东省广州市, 广州中医药大学职业技术学院. ydshy@21cn.com
电话: 020-36585519 传真: 020-36585521
收稿日期: 2005-11-23 接受日期: 2006-11-25
Apoptosis of human hepatocellular carcinoma cell line HepG2 induced by composite Gan’aining medicated serum in vitro
Dong-Sheng Yuan, Xin-Hua Wang, Chang-Qing Li, Ni Liu
Dong-Sheng Yuan, Professional Technology College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Xin-Hua Wang, Headmaster’s Office, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Chang-Qing Li, Ni Liu, Tropical Medicine Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Supported by Department of Science and Technology of Guangdong Province, No. 2005B33001032
Correspondence to: Dong-Sheng Yuan, Professional Technology College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China. ydshy@21cn.com
Received: 2005-11-23 Accepted: 2005-11-25
Abstract
AIM:To investigate the efficacy of compsite Gan’aining (CGN) in inducing the apoptosis of human hepatocellular carcinoma (HCC) cell line HepG2 in vitro.
METHODS: Human HCC HepG2 cells were co-cultured with non-medicated serum, 5-FU medicated serum, and different concentrations of CGN medicated serum, respectively. The apoptosis of HepG2 cells was detected by TUNEL and flow cytometry (FCM), respectively.
RESULTS: CGN medicated serum at different concentrations inhibited the growth of HepG2 cells in vitro ......
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