肝癌-1号对大鼠巨噬细胞杀伤肝癌细胞能力的影响及机制
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毕明星, 刘作金, 王华丽, 时毓君, 龚建平
中药;肝癌;巨噬细胞;杀伤能力毕明星,刘作金,王华丽,时毓君,龚建平.肝癌-1号对大鼠巨噬细胞杀伤肝癌细胞能力的影响及机制. 世界华人消化杂志2006;14(5)526-529,肝癌-1号
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毕明星, 王华丽, 重庆市奉节县中医院 重庆市 400010
刘作金, 时毓君, 龚建平, 重庆医科大学附属第二医院肝胆外科 重庆市 400010
重庆市卫生局科研资助项目(渝卫科教), No.[2002]01-2-01通讯作者: 龚建平, 400010, 重庆市渝中区临江路74号, 重庆医科大学附属第二医院肝胆外科. gongjianping11@hotmail.com
电话: 023-63849075-2101 传真: 023-63849075
收稿日期: 2005-09-29 接受日期: 2005-12-02
Effect of GanAi-1 on competence of murine macrophage in killing hepatic carcinoma cells and its mechanism
Ming-Xing Bi, Zuo-Jing Liu, Hua-Li Wang, Yu-Jun Shi, Jian-Ping Gong
Ming-Xing Bi, Hua-Li Wang, Chinese Traditional Medicine Hospital of Fengjie County, Chongqing 400010, China
Zuo-Jing Liu, Yu-Jun Shi, Jian-Ping Gong, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China
Supported by the Scientific Foudation from Health Department of Chongqing, No. [2002]01-2-01
Correspondence to: Jian-Ping Gong, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing University of Medical Sciences, 74 Linjiang Road, Chongqing 400010, China. gongjianping11@hotmail.com
Received: 2005-09-29 Accepted: 2005-12-02
Abstract
AIM: To investigate the effect of GanAi-1 on the competence of macrophages’ killing HepG2 cells in Wistar rats, and to explore its possible mechanism.
METHODS:After isolation from Wistar rats, the macrophages were treated with different concentrations of GanAi-1 (0, 25, 50,75, 100 mmol/L) for 24 h, and the killing effect of the macrophages on HepG2 cells was evaluatedby MTT assay. The mRNA expression of rat macrophage cytokine tumor necrosis factor-a (TNF-a), nuclear factor-kB (NF-kB) and inducible nitric oxide synthase (iNOS) was detected by reverse transcription polymerase chain reaction (RT-PCR).
RESULTS:The inhibitory rates of rat macrophages on the growth of HepG2 cells were increased with the elevated concentrations of GanAi-1 and the rates were 28 ......
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