人肝细胞癌细胞亚群的克隆分离及异质性机制的初步研究
http://www.100md.com
颜 政, 方驰华
肝癌;肿瘤异质性;肿瘤干细胞颜政,方驰华.人肝细胞癌细胞亚群的克隆分离及异质性机制的初步研究. 世界华人消化杂志2006;14(5)481-4850引言,人肝细胞癌细胞亚群的克隆分离及异质
 |
| 第1页 |
参见附件(878KB，5页)。
颜政,方驰华, 南方医科大学珠½医院普外科 广东省广州市 510282
颜政, 1963年生,男, 湖南人, 汉族,南方医科大学博士生, 副教授,主要从事肿瘤外科治疗、干细胞培养等方面基础与临床研究工作.
广东省自然科学基金资助课题, No. 2002-020097
通讯作者: 方驰华, 510282, 广东省广州市, 南方医科大学珠½医院普外科. fch58520@sina.com
电话: 020-61643211 传真: 020-61643020
收稿日期: 2005-11-08 接受日期: 2005-11-24
Elementary study on subpopulation cell isolation and tumor heterogeneity of human hepatocellular carcinoma
Zheng Yan, Chi-Hua Fang
Yan Zheng, Fang Chi-Hua, Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
Supported by Natural Science Foundation of Guangdong Province, No. 2002-020097
Correspondence to: Chi-Hua Fang, Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province,
China. fch58520@sina.com
Received: 2005-11-08 Accepted: 2005-11-24
Abstract
AIM: To isolate and characterize the heterogene-ous subpopulation cells from human hepatocel-lular carcinoma (HCC), and to explore the mech-anism of HCC heterogeneity.
METHODS:Tumor samples were obtained from 1 patient with HCC after surgery, and then HCC cells were cultured in vitro by primary tissue culture technique. Heterogeneous subpopulations were isolated by limiting dilution and their growths were observed with the help of cell counts. The cycle and DNA content of tumor cells were investigated with flow cytometry. Each subpopulation was implanted in nude mice subcutaneously to measure the tumor forming ability.
RESULTS:Two subpopulations of HCC cells, LCSC-1 and LCSC-2, were derived from primary tumor tissues. LCSC-1 cells show a sharp and spindle feature, and could not form new tumor when implanted in nude mice (0/8). However, LCSC-2 cells were multangular in shape, with protuberances, and could produce new tumor (8/8). In comparison with LCSC-2 cells, LCSC-1 had stronger proliferation ability, and the double time (18 ......
您现在查看是摘要介绍页，详见PDF附件(878KB，5页)。