再灌注损伤_____再灌注损伤；

重组人促红细胞生成素对骨骼肌缺血再灌注损伤的保护作用

http://www.100md.com 《中国矫形外科杂志》 2005年第21期

再灌注损伤,,再灌注损伤；,骨骼肌；,重组人促红细胞生成素,1材料和方法,2结果,3讨论,参考文献：

     摘要：[目的]观察重组人促红细胞生成素(rHuEPO)对肢体骨骼肌缺血再灌注(I/R)损伤的保护作用。[方法]建立大鼠后肢缺血再灌注模型。40只大鼠随机均分为：假手术组(Ⅰ组)，I/R组(Ⅱ组)，I/R+生理盐水组(Ⅲ组)，I/R+rHuEPO组(Ⅳ组)。取血浆测定丙二醛(MDA)、肌酸磷酸激酶(CPK)和乳酸脱氢酶(LDH)含量。取骨骼肌标本测定髓过氧化酶(MP0)活性、湿重/干重比(Wet/dry)。[结果]Ⅱ组与Ⅰ组比较，血浆和骨骼肌的各项生化指标显著增高(P<001)；Ⅳ组血浆及骨骼肌各项测定指标较Ⅱ组相比明显降低(P<001)。Ⅲ组和Ⅱ组之间比较，差异无显著意义。[结论]rHuEPO对肢体骨骼肌缺血再灌注损伤有保护作用。

    关键词：再灌注损伤；骨骼肌；重组人促红细胞生成素

    Protective effect of recombinant human erythropoietin on ischemia/reperfusion injury of rat skeletal muscle

    LIU Ya,CAI Jinfang,LI Zhengui,et al

    Department of Orthopedic Surgery,General Hospital of Jinan Military Command,Jinan 250031

    Abstract：[Objective]To observe the protective effect of recombinant human erythropoietin(rHuEPO)on ischemia/reperfusion(IR)injury of skeletal muscle in the limbs of rats[Method] After establishing the rat model of hind limb ischemia/reperfusion,forty rats were divided into 4 groups:sham operation group(Group Ⅰ),I/R group(Group Ⅱ),I/R + normal saline treatment group(Group Ⅲ),I/R + rHuEPO treatment group(Group Ⅳ)The levels of malodialdehy(MDA), creatinephosphokinase(CPK)and lactatedehpdrogesase(LDH)in plasma and myeloperoxidase(MPO)ratio,wet/dry of skeletal muscle were measured respectively[Result]The indexes of plasma and skeletal muscle in group Ⅱ were significantly higher than those in groupⅠ(P<001),while the indexes of the group Ⅳ were significantly lower than those in group Ⅱ(P<001) ......

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