Inhibition of proliferation and induction of differentiation of leukemia cell line HL60 by glucosamine sulphate

    DONG Baoª²Xia, WANG Zhe, LIANG Rong£¬ CHEN Xieª²Qun, HUANG Gaoª²Sheng, QIAO Yan£¬ WANG Aiª²Qin

    1Department of Hematology, Xijing Hospital, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China, 3Lanzhou Chemistry Physics Institute, Chinese Academy of Science, Lanzhou 730000, China

    ¡¾Abstract¡¿ AIM: To study the differentiation and inhibition of the proliferation of leukemia cell line HL60 induced by glucosamine sulphate (GS). METHODS: Using trypanblue, growth curves were drawn by vital cells number counting. Wrightª²Giemsa staining was performed to observe the morphology changes of HL60 cells and flow cytometry was used to observe cell cycles and the expression of CD11b£¬CD14£¬ CD13 and CD33, separately. RESULTS: The changes of the growth curve showed that the proliferation of HL60 cells was inhibited by GS. After treatment with 5 mmol/L GS for 48 hours, the percentage of G1 phase HL60 cells increased more than that of the untreated and the percentage of S phase cells decreased. No subª²G1 was observed. After treated with 1 mmol/L or 5 mmol/L GS for 120 hours, the expression of CD11b and CD14 increased, but no significant changes of CD13 and CD33 expression were observed. CONCLUSION: GS can inhibit the proliferation of HL60 cells and induce the differentiation of HL60 cells along the granulocytic or monocytic lineage and the mechanism needs to be further studied. ......