Email.liushuyuan2001@163.comEffect of ROSª²generating system on nonenzymatic protein glycation

    LIU Shuª²Yuan, HAI Chunª²Xu, ZHAO Kangª²Tao

    Department of Toxicology, School of Preventive Medicine, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM£º To investigate the effect of ROSª²generating system on nonenzymatic protein glycation. METHODS: Nonenzymatic protein glycation reactive solutions were established by Horinchi methods and divided into control groups and ROSª²generating groups. Different dosage of H2O2, CHP and Vc/Fe2+ was added into ROSª²generating groups, and then all groups were kept at 37¡æ for 60 days. Fluorescence value of AGEs products identified by fluorescence spectrum scanning was measured at EX 370 nm/EM 440 nm. RESULTS: Fluorescence spectrum scanning showed that the peak was at EM 440-450 nm. Fluorescence value in all ROSª²generating groups was significantly lower than that in control group (P<0.05). In H2O2 group, the fluorescence value changed according to different final concentration, but there was no significant difference between 20 ¦Ìmol/L and 80 ¦Ìmol/L groups (P>0.05). Between CHP and Vc/Fe2+ ROSª²generating groups, the fluorescence value was significantly different (P<0.05). CONCLUSION: This study indicated that nonenzymatic protein glycation could be inhibited by ROSª²generating system with H2O2, CHP and Vc/Fe2+. Also, there is a doseª²effect relationship between H2O2 final concentration and nonenzymatic protein glycation inhibition. ......