Study of biological characteristics of microcarrierª²attached SD rat hepatocytes

    XU Zhe, BAI Xueª²Fan, TENG Guangª²Ju, HUANG Changª²Xing, LI Yu

    Center of Diagnosis and Treatment for Infectious Diseases of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi¡¯an 710038, China

    ¡¾Abstract¡¿ AIM: To explore the biological characteristics of microcarrierª²attached SD rat hepatocytes. METHODS: The SD rat hepatocytes obtained by collagenase digestion were cultured on microcarrier. The morpholgical changes were examined by reverse microscopy and scanning electron microscopy, and the levels of albumin, LDH, urea were detected by CLª²800 auto biochemical analysis. RESULTS: The morphological characteristics and abilities of albumin and urea synthesis remained unchanged for more than 1 week. The LDH leakage, albumin synthesis and urea level were found to be fluctuated within one week£¬and there was a less LDH leakage and higher albumin and urea levels on the third day. CONCLUSION: Microcarrier culture can provide hepatocytes with high density and viability. Microcarrierª²attached hepatocytes canbe a good cell material for hepatocyte transplantation and bioartificial liver support system (BLSS). As the microcarrierª²attached rat hepatocytes have the best function on the third day, the third day may be the optimal time of applying microcarrier culture hepatocytes for hepatocyte transplantation and BLSS. ......