Biocompatibility of RP manufactured PDLLA/TCP tissue engineered bone scaffolds in low dose PTHª²induced bone anabolism in vitro

    MA Xing, HU Yunª²Yu, YAN Yongª²Nian, XIONG Zhuo, L¨¹ Rong, WANG Jun, XU Xinª²Zhi, BAI Jianª²Ping

    1PLA Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 2Laser Rapid Prototyping Biomanufacturing Center, School of Mechanical Engineering, Tsinghua University, Beijing 100084, China

    ¡¾Abstract¡¿ AIM: To investigate the cell biocompatibility of tissue engineered bone scaffolds in lowª²dose parathyroid hormone (LDª²PTH)ª²induced bone formation activities in vitro. METHODS: Tridimensional PDLLA/TCP scaffolds (with component ratio of 7¡Ã3) were synthesized by RP and observed by scanning electron microscopy. Human bone marrow stromal cells (MSCs) were primarily cultured and divided into four groups according to the different mode of exposure. The four groups were: Group A, control culture group; Group B: PDLLA/TCP immersed medium culture group; Group C: LDª²PTH [10 nmol/L hPTH (1-34)] intermittent exposure group; and Group D: LDª²PTH+PDLLA/TCP immersed medium culture group. A 48 h incubation cycle was repeated 8 times in 4 groups and the cell proliferation, gap junctional intercellular communication (GJIC) and bone like formation activities in vitro were detected by number of cell doubling (NCD), Lucifer Yellow (LY) scrape loading dye transfer (SLDT) and Von Kossa staining, respectively. RESULTS: The PDLLA/TCP scaffolds established by RP technology had good biomaterial properties such as satisfactory microstructure and porosities. The PDLLA/TCP immersed culture medium had no significant negative effects on cell growth (P>0.05) and functional differentiation in the presence of LDª²PTH. CONCLUSION: PDLLA/TCP composite scaffolds established by RP have ideal microstructure, porosities and cell biocompatibilities in LDª²PTH stimulated bone formation activities. ......