软骨细胞__(期刊论文)

骨炎定方对一氧化氮抑制人骨关节炎软骨细胞Ⅱ型胶原合成的影响

http://www.100md.com 《中国矫形外科杂志》 2005年第23期

软骨细胞,,中医药；补肾益气行血法；骨炎定；软骨细胞；一氧化氮合酶；Ⅱ型胶原,1材料与方法,2结果,3讨论,参考文献：

     摘要：[目的]观察骨炎定方对一氧化氮抑制离体人骨关节炎软骨细胞Ⅱ型胶原合成的影响，探讨中医药补肾益气行血法保护软骨细胞的作用途径。[方法]髋关节炎行关节置换术中获取离体股骨头关节软骨，行软骨细胞分离培养传代，通过设立空白血清对照组，IL1组、IL1加NAME组、补肾益气行血法中药骨炎定方含药血清组加入传代软骨细胞，采用MTT法于不同时间测定软骨细胞增殖影响，并运用逆转录聚合酶链技术检测对软骨细胞一氧化氮合酶(iNOS)和Ⅱ型胶原mRNA的影响。[结果]骨炎定方含药血清组在不同时间段均有促进软骨细胞增殖的作用。和对照组相比，3组(IL1组、IL1加NAME组、IL1+骨炎定方含药血清组)均明显了增加iNOS mRNA的表达，但加入NAME组及骨炎定方含药血清组和IL1组相比，可以抑制iNOS mRNA的表达，和IL1组相比，有明显的差异性(P<005)。和IL1组相比，3组(对照组、IL1加NAME组、IL1+骨炎定方含药血清组)均明显增加了Ⅱ型胶原mRNA的表达，但各组间无差异(P>005)。培养细胞上清液中亚硝酸盐含量测定显示：关节软骨细胞几乎不产生NO，但在IL1刺激下可产生NO，而加入NO合酶抑制剂NAME后，可部分抑制NO产生，骨炎定方含药血清组也显示出可以部分抑制NO的产生，其作用与NO合酶抑制剂NAME统计学处理没有明显差异。[结论]骨炎定方可以促进人软骨细胞增殖、能抑制iNOS的表达，增加Ⅱ型胶原的表达，这种作用与使用一氧化氮合酶抑制剂得出的结果没有明显差异，可能是中医药补肾益气行血法保护软骨细胞作用途径之一。

    关键词：中医药；补肾益气行血法；骨炎定；软骨细胞；一氧化氮合酶；Ⅱ型胶原

    Effect of Chinese Medicine gu yan ding on inhibition of AO chondrocyte secreting collagen type Ⅱ by nitrogen monoxidum

    ZHENG Xiaohui,ZHOU Qishi,WANG Haibin,et al

    Department of Orthopaedics,The First Affiliated Hospital,Guang Dong University of TCM,Guangzhou,Guangdong 510405

    Abstract：[Objective]To observes the effect of Chinese medicine gu yan ding (GYD)on the inhibition of AO chondrocyte secreting collagen type Ⅱ by nitrogen monoxidum,and to study its protecting mechanism[Method]Chondrocytes were isolated from AO femoral head cartilage and divided into control group of pure blood serum,IL1 group,IL1+NAME group,GYD+IL1 groupGYD was added to subculture cells and its influence on chondroncyte proliferation in different time was examined by MTT method,and the influence on collagen type Ⅱ and iNOS (inducible nitrogen oxide synthase),and mRNA expression were also examined by RTPCR method[Result]GYD could proliferate chondrocyte in different stage,the iNOSmRNA expression was higher in the experimental groups than that in control groupThe expression was inhibited in IL1+NAME group and GYD+IL1 group,as compared with IL1 group with a significant difference (P<005)The collagen type Ⅱ expression was higher in all three experiment groups than that in control group (P>005)without significant differenceNitrite content test in supernatant appeared that chondrocyte almost did not secret NO,but secreted NO after IL1 stimulating,and secret was inhibited after adding NOS inhibitor without significant difference[Conclusion]GYD can stimulate proliferation of chondrocyte,inhibit iNOS,increase collage type Ⅱ,and iNOSIt plays a role in the mechanism of chondrocyte protection ......

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