Effects of Epsteinª²Barr virus protein on production of B cellsª²cultured TNFª²¦Á and ILª²8

    ZHANG Yanª²Guo£¬ WANG Changª²Jun£¬ LIU Yuª²Feng£¬ GAO Tianª²Wen£¬ PAN Lei

    1Center of Dermatology of Chinese PLA, Xijing Hospital, 2Office of Academic Affairs, Division of Scientific Research Administration, Fourth Military Medical University, Xi¡¯an 710033, China, 3Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi¡¯an 710038, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of EBV protein on the production of TNFª²¦Á and ILª²8 cultured by human umbilical cord blood B cells. METHODS: B cells were isolated from umbilical blood, treated respectively with UV and heatª²inactivated EBV, and then they were cultured in complete IMDM. CD5, CD3, CD4 and CD8 cells were measured by flow cytometric analysis. TNFª²¦Á and ILª²8 in the supernatant were detected by ELISA. RESULTS: No CD3, CD4 or CD8 cells were observed and CD5+ B cells account for 43% of all the detected cells at the 14th day, and 47% at the 28th day. Significant difference of the A value of TNFª²¦Á in UVª²inactivated EBV group was observed at 2 d, 18 d, 22 d and 26 d time points (P<0.05) and significant difference of ILª²8 was observed 18 d, 22 d, 26 d and 30 d time points (P<0.05). But no significant difference of TNFª²¦Á and ILª²8 A value was observed in heatª²inactivated EBV group(P>0.05). CONCLUSION: UVª²inactivated EBV is able to induce TNFª²¦Á and ILª²8 production while heatª²inactivated EBV can not. The results suggest that EBV protein may exert some influence on the production of inflammatory factors. ......