【摘要】 目的 探讨端粒酶作为一种新的生物学标志物用于常见胸部恶性肿瘤诊断和预后评价等方面的临床应用价值。方法 采用端粒重复序列扩增法对原发性肺癌、食管癌手术切除组织及相应的癌旁组织进行端粒酶活性分析，并以相应的良性病变作为对照。结果 32例肺癌组织中端粒酶阳性率为84.4%，癌旁组织为9.4%，在良性组织中未检出端粒酶阳性。端粒酶活性随肺癌临床分期有升高趋势，伴淋巴结转移标本组中其阳性率明显高于非淋巴结转移组(P<0.05)，但与肿瘤病理组织学类型、分化程度等差异无显著性(P>0.05)。37例食管癌端粒酶阳性率为89.12%，癌旁组织为18.92%，正常食管组织未检出端粒酶阳性。当癌侵及肌层及外侵后，其阳性表达率高于局限于黏膜及黏膜下层者，差异有显著性(P<0.01)。伴淋巴结转移标本中其阳性率高于非淋巴结转移者(P<0.05)。结论 端粒酶有可能成为肺癌、食管癌早期诊断和判断预后的重要生物标志物和治疗新靶点。

    【关键词】 肺肿瘤；食管肿瘤；病理学；端粒酶；诊断；生物治疗

    Telomerase activity in common thoracic malignant tumors

    SHI Hong-can，SHI Wei-ping，SHU Yu-sheng，et al.

    Yangzhou University College of Clinical Medicine，Yangzhou 225001，China

    【Abstract】 Objective To investigate the correlation between the expression of telomerase activity and the clinicopathological characteristics of common thoracic malignant tumors.Methods Lung and esophageal carcinomas and corresponding tissues adjacent to carcinomas were obtained from 69 surgically treated，and benign tissues were used to be controls.The PCR-based telomeric repeat amplification protocal-enzyme linked immunosorbent assay was conducted for detection of telomerase activity in these samples.Results Telomerase activity was detected in 27(84.4%)of 32 lung carcinomatous tissues ......