Effects of azone on transdermal absorption of ginsenoside Rg1 and minoxidil

    ZHOU Min, GU Yi, ZHANG Sanª²Qi, XIA Ruª²Shan, FANG Kunª²Quan

    1Department of Pharmacy, 2Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To study the proper mass concentration of azone used as the permeator of ginseng minoxidil preparation. METHODS: Using a device of transdermal diffusion and using the mouse skin in vitro as barrier and 9 mg/L NaCL as accepted medium, we studied the effects of azone with different concentrations on transdermal absorption. RPª²HPLC method was adopted to measure the amount of transdermal absorption of ginsengª²minoxidil preparation. RESULTS: The amount of transdermal absorption of ginsenoside Rg1 within 24 h was (0.26¡À0.02), (3.47¡À0.16), (3.98¡À0.23), (4.54¡À0.73), (2.36¡À0.11) and (1.65¡À0.08) ¦Ìg/mL in 0, 10, 30, 50, 70 and 90 mL/L azone groups, respectively. The amount of transdermal absorption of minoxidil within 24 h was (8.87¡À0.18), (11.21¡À0.20), (12.73¡À0.36), (11.72¡À0.36), (7.22¡À1.29) and (4.65¡À0.26) ¦Ìg/mL in 0, 10, 30, 50, 70 and 90 mL/L azone groups, respectively. CONCLUSION: 30 mL/L is the optimal concentration for azone used as a transdermal enhancer in percutaneous absorption of ginseng and minoxidil through mice skin in vitro. ......