Epidemiological survey of cryptosporidiosis in Anhui province

    LI Chaoª²Pin, WANG Keª²Xia, WANG Jian, PAN Boª²Rong

    1Department of Aetiology & Immunology, College of Medicine, Anhui University of Science & Technology, Huainan 232001, China, 2Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To provide scientific evidence for prevention and controlling of cryptosporidiosis, the infection of Cryptosporidium parvum and its epidemiological characteristics were studied in some areas of Anhui province. METHODS: The oocyst of Cryptosporidium parvum in 5421 fresh stool samples from eleven areas of Anhui province was tested by auramineª²phenol stain and improved antiª²acid stain respectively. The specific antibody of IgG, IgM and T subsets of 41 patients with positive Cryptosporidium parvum in stools were detected by ELISA and biotinª²streptavidin (BSA) respectively. RESULTS: The total infective rate of Cryptosporidium parvum was 1.33%. Among them, the positive rates of oocyst in the areas of Huaibei (1.82%) and Fuyang (1.80%) were higher. The positive rates of oocyst in stools of infants, pupils, middle school students, college students, adults, patients with diarrhea, and those with immunodeficiency were 3.15%, 0.82 %, 0.82%, 0.83%, 0.85%, 2.88% and 8.33% respectively. The positive rates of oocyst in infants and the patients with diarrhea and immunodeficiency were significantly higher than those in others (P<0.01). The positive rate of oocyst in males was similar to that in females (P>0.05). The positive rate of oocyst in urban areas (1.13%) was significantly lower than those in rural areas (1.72%, P<0.01). The positive rates of specific IgG, IgM and IgG+IgM in sera of the patients with positive oocyst in stool were 63.4%, 17.1% and 19.51% respectively. The number fractions of T subsets of CD3+, CD4+, CD8+ and CD4+/CD8+ of the patients were (0.66¡À0.07)% ......