人胸椎_____人胸椎；黄韧带；

人胸椎黄韧带BMP2和TGFβ的基因表达增龄性变化

http://www.100md.com 《中国矫形外科杂志》 2005年第19期

人胸椎,,人胸椎；黄韧带；,BMP2和TGFβ;,基因表达,1材料和方法,2结果,3讨论,参考文献：

     摘要：[目的]研究不同年龄组胸黄韧带中BMP2和TGFβ基因表达规律。[方法]利用BMP2和TGFβ cDNA探针，对不同年龄组的黄韧带冰冻切片进行原位杂交，用VIDAS图像分析仪分析。[结果]20岁以下组，有明显的TGFβ表达，无BMP2表达；21~40岁组，黄韧带的附着点处有少量的BMP2和TGFβ阳性表达信号。41~60岁组，在附着点处TGFβ表达较弱，但BMP2表达相对增强。61岁以上组，TGFβ无表达，附着点处有呈簇状BMP2阳性表达信号。VIDAS图像分析显示各组间差异有显著性。[结论]随着年龄的增长，TGFβ表达逐渐减弱，在附着点处出现BMP2的表达。

    关键词：人胸椎；黄韧带； BMP2和TGFβ; 基因表达

    Gene expression of collagen BMP2 and TGFβ in human lumbar ligamentum flavum

    GU Shuming,TIAN Jiwei,XU Lin,et al

    Department of Orthopedics,the Affiliated Hospital of Beijing Traditional Chinese Medicine University,Beijing 100700

    Abstract：[Objective]To observe the gene expression of collagen BMP2 (bone morphogenetic protein2) and TGFβ (transforming growth factorβ) in human thoracic ligamentum flavum[Method]BMP2 and TGF13 probes were labeled and in situ hybridization results were analyzed by VIDAS system[Result](1) Under 20 years group:the positive gene expression of TGFβ was evident,but it was negative to BMP2(2) 21 ~40 years group:there were a few positive signal of both BMP2 and TGFβ on the insertion part(3) 41 ~60 years group:there was only few TGFβ positive signal on insertion part,but BMP2 gene expression was relatively high(4) Over 61 years group:there was not TGFβ expression,but BMP2 expression could only be seen on insertion part and junction part,and was low,but the cells were areatus[Conclusion]In normal ligamentum,there is expression of TGFβ gene,but it decreases with age;with age advancing BMP2 emerges and enhances in the insertion part of ligamentum flavum ......

百拇医药网 http://www.100md.com/html/DirDu/2006/02/19/87/16/34.htm

您现在查看是摘要页，全文长 6504 字符。