寡核苷酸类__(期刊论文)

反义cmyc寡核苷酸对大鼠气道平滑肌细胞周期的阻滞作用

http://www.100md.com 《第四军医大学学报》 2004年第6期

寡核苷酸类,,基因，myc；寡核苷酸类，反义；肌，平滑；细胞周期,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Inhibitive effect of antisense oligonucleotide for cmyc on rat airway smooth muscle cell cycle

    LIU YingGe, QI HaoWen, LI HuanZhang, SU MingQuan, YU WenBin, MA YueYun

    1Department of Respiratory Medicine, 2Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To observe the effects of antisense oligouncleotide for cmyc on rat airway smooth muscle cell cycle. METHODS: Rat airway smooth muscle cells were primarily cultured and lipofectin was used to introduce antisense oligonucleotide, sense oligonucleotide and mismatched oligonucleotide for cmyc into rat airway smooth muscle cells. Cell cycles were measured by flow cytometry and the expression of cyclin D1 and cyclin E was detected immunohistochemically. RESULTS: Antisense oligonucleotide for cmyc inhibited rat airway smooth muscle cells through S phase. Sense and mismatched oligonucleotides exerted no effect on cell cycles. Antisense oligonucleotide for cmyc decreased the expression of cyclin D1 and cyclin E. CONCLUSION: Antisense oligonucleotides for cmyc inhibit rat airway smooth muscle cells through S phase and decrease the expression of cyclin D1 and cyclin E. ......

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