真核分泌表达__(期刊论文)

Nogo66受体LRR功能区的真核分泌表达

http://www.100md.com 《第四军医大学学报》 2004年第2期

真核分泌表达,,Nogo66受体；LRR；真核分泌表达,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Secretory expression of leucinerich repeats of Nogo66 receptor in eukaryotic cells

    ZHAO XiangHui, JIN WeiLin, LI Rong, Jü Gong

    PLA Institute of Neurosciences, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To construct the eukaryotic secretion vector containing the coding region of human Nogo66 receptor LRR domain and to detect its expression in COS7 cells. METHODS: LRR gene, derived from a prokaryotic plasmid pESHis/LRR, was subcloned into a eukaryotic secretion vector pSectag2B. The recombinant expression plasmid, pSectag2B/LRR, was transfected into COS7 cells with lipofectamine 2000 reagent. RESULTS: Expression of a secreted fusion protein was identified by immunoflurescence assay, Westernblot analysis and sandwich ELISA assay with anticMyc mAb and antiHis antibody. CONCLUSION: Eukaryoric expression vector containing the LRR gene was successfully constructed and expressed in COS7 cells.

    【Keywords】 Nogo66 receptor; LRR; eukaryotic expression system

    【摘要】目的：构建含有人Nogo66受体LRR功能区段cDNA序列的真核分泌型表达载体 ......

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