Collection and purification of CD34+ hematopoietic stem (progenitor) cells and its subª²groups from patients with multiple sclerosis

    QIN Chao, MO Xueª²An, LU Rui, LING Li, MO Wuª²Ning

    1Department of Neurology, 2Department of Clinical Laboratories, First Affiliated Hospital, Guangxi Medical University, Nangning 530021, China

    ¡¾Abstract¡¿ AIM: To collect and purify the bone marrow CD34+ hematopoietic stem cells and its subª²groups from the patients with multiple sclerosis. METHODS: CD34+ cells were collected and purified by means of imunomagnetic beads on MiniMACS device. Flow cytometry was emplyed to determine percentage of CD34+ cells, CD34+/CD38- cells and CD34+/CD38+ cells before and after miniMACS. RESULTS: The purity of CD34+ cells amounted to more than 91% after the purification. The purity of CD34+/CD38- cells and CD34+/CD38+ cells amounted to more than 98% after fluorescent activated cell sorter (FACS). CONCLUSION: The combination of MiniMACS and FACS can sort effectively the bone marrow CD34+ cells and its subª²groups so that they may be used for hematopoietic stem cell transplantation, gene therapy and biological study of hematopoietic stem cells/hematopoietic progenitor cells (HSC/HPC) and its subª²groups. ......