胃肿瘤_____胃肿瘤；细胞分裂；氯化物通道；膜片钳术

容量调控氯通道在人胃癌细胞系SGC7901增殖中的作用

http://www.100md.com 《第四军医大学学报》 2004年第6期

胃肿瘤,,胃肿瘤；细胞分裂；氯化物通道；膜片钳术,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Role of volumeregulated chloride channel in proliferation of human gastric carcinoma cell line SGC7901

    CHEN YanMing, YANG Jun, HU YuZhen, TIAN Jing, ZHOU ShiSheng

    Department of Physiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study the role of volumeregulated chloride channel in the proliferation of human gastric carcinoma cells. METHODS: Cell proliferation was analyzed by cell counting and MTT assay and the cell cycle analysis was carried out by flow cytometry. The currents of volumeregulated chloride channel were recorded by patch clamp. RESULTS: NPPB (100 μmol/L), a chloride channel blocker, reversibly decreased the cell number and the MTT optical density (OD) of SGC7901. Compared with control, NPPB arrested cells in G1 phase and extracelluar application of NPPB inhibited volumeregulated chloride currents which were induced by hypotonic stress. CONCLUSION: The volumeregulated chloride channel plays an important role in the proliferation of gastric carcinoma cell. ......

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