Construction and identification of recombinant adenovirus containing chimeric gene G2S0.7 of Hantaan virus

    ZHANG Fangª²Lin, LIU Yong, YU Lan, HU Gang, WU Xingª²An, SHI Mengª²Yuan, BAI Wenª²Tao, WANG Haiª²Tao, XU Zhiª²Kai

    Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To express the chimeric gene containing M segment and 0.7 kb fragment of S segment of Hantaan virus in adenovirus expression system. METHODS: The recombinant adenovirus transfer vector pShuttleª²G2S0.7 was constructed and the chimeric gene was inserted into adenovirus DNA. After transfecting HEK293 cells, the recombinant adenovirus was obtained and the titer of it was determined and the expressed product was detected by ELISA. RESULTS: The recombinant adenovirus containing the chimeric gene G2S0.7 was constructed successfully and the titer of it was about 1013ª²1015pfu/L. HEK293 cells were then infected by the recombinant adenovirus and fusion protein could be expressed in the infected cells. The expressed protein could be recognized by the Hantaan virus NPª²specific mAb and glycoprotein G2ª²specific mAb. CONCLUSION: The successful expression of G2S0.7 with biological activity in adenovirus expression system lays the basis for further research on its immunological characteristics. ......