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    Cloning high expression and location of H.pylori adhesion babA2 gene

    BAI Yang, WANG Jiª²De, CHEN Ye, LIN Huanª²Jian, ZHANG Zhaoª²San, ZHANG Yaª²Li

    1PLA Institute of Digestive Diseases, First Military Medical University, Guangzhou 510515, China, 2Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China

    ¡¾Abstract¡¿ AIM: To isolate and express babA2 gene from H.pylori. METHODS: babA2 gene was amplified from H.pylori chromosome by PCR and inserted into the prokaryotic expression vector pETª²22b(+).The recombinant plasmid was transformed into the BL21(DE3) E.coli strain. RESULTS: The 2.2 kb babA2 gene was successfully isolated. Recombinant E.coli strains expressed babA2 were obtained, the expression protein amounted to 34.8% of the total bacterial protein , 22.7% of the bacterial periplasm protein , 15.0% of the bacterial sonicate supernatant and 86.7% of the bacterial inclusion body after being induced with IPTG for 3 h at 37¡æ. CONCLUSION: Cloning and highª²expression of babA2 gene lay the foundation for further research in the molecular mechanism of H.pylori adhesin and constructing the H.pylori vaccine. ......