脑缺血_____脑缺血；再灌注；DNA单链断裂；

类缺血再灌注小鼠皮质神经元DNA单链损伤与Bax表达的关系

http://www.100md.com 《第四军医大学学报》 2003年第7期

脑缺血,,脑缺血；再灌注；DNA单链断裂；,Bax；,Klenow法,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学西京医院神经内科，陕西西安 710033)

    Relationship between DNA singlestrand damage and Bax expression in mouse cerebral cortical neurons under ischemiclike conditions

    YU YingXin,Wan Qi, ZHANG RuiGuo, SUN WenPing

    Depattment of Neurology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study DNA singlestrand damage at different time points and expression of Bax in cortical neurons under ischemiclike conditions and to investigate the relationship of the expression of Bax and DNA singlestrand damage. METHODS: The expression of Bax was detected by flow cytometer in cortical neurons under ischemiclike conditions. The treated neurons were detected by Klenow method at different time point of reperfusion for the positive expression and a half quantitative analysis was made. RESULTS: 2 hours after reperfusion, the expression of Bax reached peak point and the positive neurons of Bax decreased with the increase of reperfusion time. The average gray of Klenow positive cells with no reperfusion was 72.4±1.0 and increased with the increase of reperfusion time. The highest average gray was 135.5±1.3 at 2 hour of reperfusion. The ratio of Bax positive cells was related to the average gray of Klenow positive cells. CONCLUSION: Under ischemiclike conditions, the expression of Bax reaches peak point 2 hours after reperfusion and so is the DNA singlestrand damage, suggesting that the expression of Bax may be related to the DNA singlestrand damage. ......

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