Induction of secretion of MCPª²1 from epithelial cells by proteinase activated receptorsª²2 agonists

    WANG Haiª²Yan, HE Shaoª²Heng

    Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515031, China

    ¡¾Abstract¡¿ AIM: To investigate the actions of agonists of proteinase activated receptors (PAR)ª²2 on the secretion of monocyte chemoattractant proteinª²1 (MCPª²1) from human lung epithelial cells. METHODS: A549 cells were cultured in a 12ª²well culture plate. The challenge was performed by adding various concentrations of PARª²2 agonists or their reverse peptides into each well, respectively. After 2 h, 8 h or 16 h, the reactions were terminated by removal of the supernatant from each well. A Sandwich ELISA was used to determine the levels of MCPª²1 in supernatants. RESULTS: Following 16 h incubation, PARª²2 agonists Serª²Leuª²Ileª²Glyª²Lysª²Valª²amide (SLIGKV) and transª²cinnamoylª²Leuª²Ileª²Glyª²Argª²Leuª²Ornª²amide (tcª²LIGRLO) were able to induce concentration dependent secretion of MCPª²1. The maximum release of MCPª²1 was 13 fold more than the baseline release. The reverse PARª²2 agonists had little effects on MCPª²1 release. The time course showed that the actions of PARª²2 agonists initiated at 2 h and reached their peaks at 16 h. CONCLUSION: Agonists of PARª²2 are potent secretogogues of MCPª²1 release from cultured human lung epithelial cells. Their antagonists may possess the ability to inhibit airway inflammation. ......