Effect of resveratrol derived from Rhizoma polygoni Cuspidati on apoptosis of gastric cancer cells HGC27

    LI Ying, YAO Liª²Bo, WANG Liª²Feng, HAN Jiong, HAN Yueª²Heng, LIU Xinª²Ping, LIN Shuª²Xin, YU Qiang

    1Department of Biochemistry & Molecular Biology, 2Department of Pathophysiology, School of Basic Medicine, Fourth Military Medical University, Xi¡¯an 710033, China, 3Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

    ¡¾Abstract¡¿ AIM: To explore the inductive effects of the resveratrol from Rhizoma polygoni Cuspidati on the cell cycles, growth and apoptosis of the gastric cancer cells HGC27. METHODS: MTT method and soft agar assay were performed to observe the effects of the resveratrol on the cell growth. Annxinª²V & PI staining assay and FCM assay were used to examine the inductive effects of resveratrol on the cell cycles and apoptosis. RESULTS: MTT results manifested that the cell survival rate decreased significantly and in dose dependent manner 24 h after HGC27 was incubated with 0.5 mmol/L resveratrol (P<0.01 vs control). Soft agar assay results indicated that HGC27 could not form cell colony when it was incubated with 0.5 mmol/L resveratrol. Annxinª²V & PI staining assay and FCM assay showed that resveratrol could inhibit the HGC27 to G1 phase and induce cells to apoptosis (8.39%). CONCLUSION: Resveratrol can inhibit the growth of HGC27 and induce the cells to apoptosis. ......