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编号:10872292
大鼠脾脏P物质免疫反应神经纤维的分布及烫伤后含量变化
http://www.100md.com 《第四军医大学学报》 2003年第7期
烫伤,,P物质;脾脏;烫伤;免疫功能;大鼠,0引言,1材料和方法,2结果,3讨论,【参考文献】
     (第四军医大学西京医院: 神经内科, 综合诊疗中心, 陕西 西安 710033)

    Distribution and content change of SP immunoreactive nerve fibers in the rat spleen post burn

    WU ZhongLiang, REN Ning, WAN Qi

    Department of Neurology, Center of Rehabilitation, Xijing Hospital, Fourth Mililtary Medical University, Xian 710033, China

    【Abstract】 AIM: To investigate the distribution and content change of substance P (SP) immunoreactive nerve fibers in the rat spleen post burn and to explore the relation between SP and immunological functions. METHODS: The distribution of SP immunoreactive nerve fibers was observed by the immunohistochemical ABC method combined with glucose oxidaseDABnikel enhancement technique. The rats were inflicted on the back with 20% TBSA of Ⅱ degree scalding by boiling water. The post burn changes in the tissue content of the SP in the spleen were determined by radioimmunoassay (RIA). RESULTS: The rat spleen was richly innervated by positive SP immunoreactive nerve fibers. The tissue content of SP in the spleen decreased significantly (P<0.01) at 30, 60, 120 and 240 minutes post burn. CONCLUSION: The rat spleen is richly innervated by positive SP immunoreactive nerve fibers and the content of SP in the spleen decreased significantly post burn. SP may play the role of enhancing immunological function. ......

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