前列腺肿瘤__(期刊论文)

抑癌基因PTEN对人前列腺癌细胞系PC3的作用

http://www.100md.com 《第四军医大学学报》 2003年第8期

前列腺肿瘤,,基因，抑制，肿瘤；PTEN；前列腺肿瘤；PC3,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学西京医院泌尿外科，陕西西安 710033)

    Effects of tumor suppressor gene PTEN on human prostate cancer cell line PC3 in vitro

    YANG Bo, CHEN BaoQi, WANG He, SHAO Chen, LIU HeLiang, QIN RongLiang, YUAN JianLin, KANG FuXia, SHAO GuoXing

    Department of Urology, Xijing Hospital, Fourth Military Medical University, Xian 710033,China

    【Abstract】AIM：To study the effects of tumor suppressor gene PTEN on human prostate cancer cell line PC3. METHODS: Cationic liposome LipofectAmine2000 was used to transfect retrovirus vector PbabePTENpuro containing PTEN and Pbabepuro into PTENdeficient prostate cancer cell line PC3 respectively, and then cell growth, adhesion, segregation, apoptosis, cell cycle and ultrastructure alteration in vitro were studied. RESULTS: After PTEN was transfected into PC3 and expressed, cell growth rate obviously slowed down and the growth inhibition rate was 83%. Adhesion rate dropped and cell segregated from matrix more easily. FCM showed a small apoptosis peak and an obvious G1 block in cell cycle and EMS showed that cells lost some malignant features in ultrastructure. CONCLUSION: PTEN can inhibit PC3 growth and adhesion in vitro and may play an important role in prostate cancer gene therapy. ......

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