大孔吸附树脂_____山茱萸；皂苷类；大孔吸附树脂

大孔吸附树脂法分离纯化山茱萸总皂苷

http://www.100md.com 《第四军医大学学报》 2003年第8期

大孔吸附树脂,,山茱萸；皂苷类；大孔吸附树脂,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学基础部化学教研室, 陕西西安 710033，西安交通大学分离科学研究所, 陕西西安710049)

    Isolation and purification of total saponins from cornus officinalis by column chromatography of macroreticular resin

    WU Hong, LIANG Heng, WU DaoCheng, YUAN ZhongHai

    Department of Chemistry, Faculty of Preclinical Medicine, Fourth Military Medical University, Xian 710033, China, Institute of Separation Science, Xian Jiaotong University, Xian 710049, China

    【Abstract】 AIM： To optimize the extraction and refinement of total saponins from Cornus officinalis by column chromatography of macroreticular resin. METHODS：Total saponins were extracted with 700 mL·L-1 of ethanol and purified with HPD 300 macroreticular resin and eluted with distilled water, 300 mL·L-1, 500 mL·L-1 and 700 mL·L-1 of ethanol. Adsorption capability and elution parameters were observed and compared with those extracted by routine solvents. RESULTS： Total saponins from Cornus officinalis enriched in 500 mL·L-1 of ethanol eluting solution. The dosage of elution reagents was 8 times as the sample volume and the elutive ratio was 818%. The average gaining rates of the two methods were 249% and 219% respectively. CONCLUSION： It is a good method to use macroreticular resin to adsorb and purify total saponins in cornus officinalis. ......

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