Expression of p57kip2 and its relationships with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma

    GUO Hui, NAN Keª²Jun, RUAN Zhiª²Ping, JING Zhao, LIU Shaanª²Xi

    1Department of Oncology, 2Department of Hematology, First Hospital, Xi¡¯an Jiaotong University, Xi¡¯an 710061, China

    ¡¾Abstract¡¿ AIM: To investigate the expression of p57kip2 and its relationship with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma (HCC). METHODS: Expression of p57kip2, PCNA and p53 in tumor tissues from 32 patients with HCC and 10 liver tissues of normal persons was detected with Elivision immunohistochemical technique. RESULTS: p57kip2 protein positiveª²expression rate in HCC was 56.2%£¬which was lower than that in normal tissues (100%) (P=0.011). The reduced expression of p57kip2 protein correlated significantly with moderate or lowª²differentiation of tumor cells (P=0.007), high clinical stage (P=0.041) and poor prognosis (P=0.036), but did not correlate significantly with metastasis, tumor size, level of AFP and age (P>0.05). PCNA positiveª²expression rate was 56.2%, which was correlated significantly with the expression of p57kip2 (P=0.036) and p53 positiveª²expression rate was 46.9%, which was not correlated significantly with the expression of p57kip2 (P>0.05). CONCLUSION: There is a marked loss or absence of p57kip2 expression and high expression of PCNA in HCC, which are involved in the carcinogenesis and development of HCC. p57kip2 and p53 may induce apoptosis via different mechanisms. ......