β肌动蛋白启动子____

β肌动蛋白启动子指导下人bcl2转基因小鼠模型的建立

http://www.100md.com 《第四军医大学学报》 2004年第18期

β肌动蛋白启动子,,基因，bcl2;,β肌动蛋白启动子;,小鼠，转基因,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Establishment of transgenic mouse model carrying bcl2 under the direction of βactin promoter

    XUE RongLiang, WU Gang, Lü JianRui, LI Wei, LEI XiaoMing

    Department of Anesthesiology, The Second Hospital of Xian Jiaotong University, Xian 710004, China

    【Abstract】 AIM: To construct a transgenic mouse model carrying bcl2 gene by prokaryotic injection of promoterβactinbcl2. METHODS: The promoterβactinbcl2 expression vector was constructed and the vector was injected into mouse prokaryotic embryo and the DNA of transgenic mice was identified with PCR and Southern blot. RESULTS: The achievement rate of prokaryotic injection was 58%. Among the 33 newborn mice, 8 were tested to be bcl2 positive with PCR while Southern blotting identified only 4 bcl2 positive mice. CONCLUSION: The transgenic mouse model carrying bcl2 gene has been established by prokaryotic injection, which provides an effective model in studying the mechanism of cerebral ischemiareperfusion injury.

    【Keywords】 genes, bcl2; βactin promoter; mice, transgenic

    【摘要】目的：通过原核注射法将肌动蛋白启动子/bcl2基因转入小鼠受精卵 ......

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