Expression of hypoxiaª²inducible factorª²1alpha(HIFª²1¦Á)and inducible nitric oxide synthase(iNOS)genes in inflammatory bowel disease

    XU Chunª²Mei, DONG Weiª²Guo, YU Baoª²Ping, LUO Heª²Sheng, YU Jieª²Ping

    Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China

    ¡¾Abstract¡¿ AIM: To investigate the expression and action of HIFª²1¦Á and iNOS genes in inflammatory bowel diseases. METHODS: Paraffin embedded tissues from inflammatory bowel disease which had been diagnosed by clinical, endoscopic, histological and radiological examinations were used and immunohistochemistry method was performed to detect the expression of HIFª²1¦Á and iNOS genes. RESULTS: The median of HIFª²1¦Á positive expression rate was 54%, 59% and 1%, and that of iNOS expression was 58%, 54% and 2% in 47 ulcerative colitis, 13 Crohn¡¯s disease and 10 normal colon mucosa respectively. The HIFª²1¦Á and iNOS expression of ulcerative colitis and Crohn¡¯s disease was significantly different from that of normal colon mucosa (P<0.01), while no difference was found between Crohn¡¯s disease and normal colon mucosa (P>0.05). Linear correlation analysis showed that iNOS protein was positively correlated with HIFª²1¦Á protein (ulcerative colitis: r=0.627; Crohn¡¯s disease: r=0.610, P<0.05). CONCLUSION: HIFª²1¦Á and iNOS are involved in the pathogenesis of inflammatory bowel diseases and HIFª²1¦Á may be an essential regulator of iNOS gene expression. ......