Ex vivo expansion of CD34+ cells from human umbilical cord blood by adult bone marrow mesenchymal stem cells

    JIA Mingª²Feng, HOU Xiangª²Lin, ZHAO Li, LIU Bei, LI Juan, CHEN Xuan

    1Department of Hematology, 2Central Laboratory, First Affiliated Hospital, Lanzhou Medical College, Lanzhou 730000, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of adult bone marrow mesenchymal stem cells on the expansion of umbilical cord blood CD34+ cells ex vivo. METHODS: Adult bone marrow mesenchymal stem cells were isolated and cultured as feeder layer. Umbilical cord blood CD34+ cells were cultured in combination with mesenchymal stem cells, stem cell factor, Interleukinª²11 and granulocyteª²macrophage colony stimulating factor, respectively. At the end of 3 weeks, the total number of nucleated cells , colony forming cells and CD34+ cells was repeatedly counted in culture system for 3 weeks to obtain the fold of expansion. RESULTS: The mesenchymal stem cells and cytokines coª²culture systems significantly enhanced the expansion of the cord blood cells with the increase of the total number of nucleated cells by 114.2¡À2.4ª²fold, colony forming cells by 40.5¡À8.6ª²fold and CD34+ cells by 11.3¡À0.4ª²fold, respectively. CONCLUSION: The expansion of umbilical cord blood CD34+ cells can be enhanced ex vivo by adult bone marrow mesenchymal stem cells combined with cytokines. ......