Expression fingerprint analysis of chronic arseniteª²induced Lª²02 cell using cDNA microarry

    YANG Lei, GU Yongª²Qing, PAN Zeª²Min, YUAN Hongª²Lin, XIAN Lingª²Ling, WANG Guoª²Quan, YAO Hua, YING Kang, LI Yao3, XIE Yi

    1Key Laboratory of Xinjiang Endemic and Ethnologic High Incidence Diseases, Ministry of Education, Shihezi 832002, China, 2School of Public Health, Xinjiang Medical University, Urumqi 830000, China, 3Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China

    ¡¾Abstract¡¿ AIM: To investigate the expression profile of human genes in response to chronic sodium arsenite treatment by cDNA microarrays. METHODS: The RNA was purified from the Lª²02 cells without and with 4 ¦Ìmol/L arsenite sodium induction for more than 2 weeks, respectively. RESULTS: In longª²term exposure, many genes were aberrantly expressed, which included genes involved in cellª²cycle regulation, signal transduction, stress response and growthª²factor and various oncogenes. CONCLUSION: Many tumorª²related genes are aberrantly expressed when cells are exposed to arsenic environment for more than two weeks, which may offer some clues to further studies on carcinogenesis and arsenite. ......