Increased pregnant chance by conventional IVF and ICSI of sibling oocytes

    ZHANG Fengª²Ying, ZHANG Yunª²Shan, GAO Qiª²Xian, ZHANG Ying, ZHANG Meiª²Zi, WANG Xiaoª²Ru, LI Yuan

    Assisted Reproductive Technology Research Center, Tianjin Central Obstetrics and Gynecology Hospital, Tianjin 300052, China

    ¡¾Abstract¡¿ AIM: To compare and analyze the result and the clinical outcome in sibling oocytes treated by conventional inª²vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). METHODS: Sibling oocyteª²cumulus complexes (OCC) of 43 different couples were divided into two groups: IVF group and ICSI group, using the same semen sample and inseminated respectively by IVF and by ICSI (IVF+ICSI). RESULTS: In ICSI group, a total of 282 M¢ò oocytes yielded a diploid fertilization rate of 64.5%, significantly higher than that of 52.8% in the IVF group with 265 oocytes (P<0.01). Complete fertilization failure was 11.6% in IVF group but none in the ICSI group, and there was significant difference in the goodª²quality embryo rates between the IVF group (30.1%) and the ICSI group (60.8%) (P<0.01). The total cleavage rates in both IVF and ICSI groups were quite similar: 96.7% and 97.1% respectively (P<0.05). The best embryos were selected for transfer in a total of 189 embryos and of the transferred embryos, 120 embryos were in the ICSI group (63.5%) and 69 in the IVF group (36.5%). The implantation rate and clinical pregnancy rate of the sibling oocytes of IVF and ICSI (IVF+ICSI) versus independent IVF or independent ICSI were 17.5%, 10.4%, 9.8% and 41.9%, 26.2%, 28.1% respectively, which were significantly different. The take home baby rate was 77.8%. CONCLUSION: The insemination procedure of sibling oocytes by IVF and by ICSI may improve the embryo quality and increase the pregnant chance and clinical pregnant rate. ......