血小板活化因子__(期刊论文)

血小板活化因子对气道平滑肌细胞的增殖及核因子NFκB表达的影响

http://www.100md.com 《第四军医大学学报》 2004年第17期

血小板活化因子,,血小板活化因子；气道;平滑肌细胞；核因子NFκB,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effects of plateletactivating factor on proliferation of airway smooth muscle cells and expression of nuclear factorκB

    SONG YingFang， LI HuanZhang， SU MingQuan, WU ChangGui

    1Department of Respiratory Medicine， 2Department of Clinical Laboratory, Xijing Hospital， Fourth Military Medical University， Xi’an 710033, China

    【Abstract】 AIM: To investigate the effects of plateletactivating factor (PAF) on the proliferation of rat airway smooth muscle cells (ASMCs) and the expression of nuclear factorκB (NFκB). METHODS: MTT assay and flow cytometry were used to analyze the effects of PAF on the proliferation of ASMCs in vitro and NFκB activity change of the ASMCs stimulated by PAF was examined by electrophoretic mobility shift assay (EMSA). The effects of Nacetylcysteine (NAC) on the function of PAF were also studied. RESULTS: PAF (1×10-6-1×10-9 mol/L) stimulated the cell proliferation and reached its maximal effect at 1×10-7 mol/L. The proliferative index (PI) of the ASMCs significantly increased(P<0.05)and the NFκB activity was enhanced obviously(P<0.01) following stimulation of PAF (10-7 mol/L). NAC (20 mmol/L) partly inhibited the cell proliferation and the increased NFκB activity induced by PAF (P<0.05). CONCLUSION: PAF can stimulate the proliferation of ASMCs, which may be achieved partly through the activation of NFκB pathway. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/02/19/87/25/60.htm

您现在查看是摘要页，全文长 11062 字符。