Culture and neuronal induction of human bone mesenchymal stem cells in vitro

    XU Yuª²Ming£¬ QIN Jie, SONG Bo£¬ SONG Yongª²Ping, XING Ying£¬ YAN Fuª²Ling, ZHANG Suª²Ming

    1Department of Neurology, First Hospital, Zhengzhou University, 2Tumor Hospital of Henan Province, 3Stem Cells Research Center, Medical College, Zhengzhou University, Zhengzhou 450052, China, 4Department of Neurology, Zhongda Hospital, Dongnan University, Nanjing 210000, China, 5 Department of Neurology, Tongji Hospital, Huazhong Science and Technology University, Wuhan 430030, China

    ¡¾Abstract¡¿ AIM: To investigate the conditions for the neuronal induction of bone mesenchymal stem cells (MSCs) from adult human. METHODS: The MSCs were isolated primarily from bone marrow of adult volunteers¡¯ iliac and purified by passage culture. The 3rd to 5th passage of MSCs were planted into plastic petridishes and induced by 2ª²mercaptoethanol (BME) and dimethyl sulfoxide (DMSO). Immunocytochemistry was used to identify cell types. RESULTS: After a 1ª²hour induction, some of the MSCª²derived cells expressed nestin, a neural stem cell marker. After a 4ª²hour induction, a large number of cells displayed classical neuronª²like morphology and expressed neurofilament proteinª²M (NFª²M), and some of the cells exhibiting neuronal morphologies expressed microtube associated proteinª²2 (MAPª²2), which was a neuron marker. CONCLUSION: MSCs from adult human can be cultured, proliferated and differentiated into neuronª²like cells in vitro. ......