膀胱肿瘤__(期刊论文)

尿脱落细胞6号染色体长臂的微卫星改变诊断膀胱肿瘤

http://www.100md.com 《第四军医大学学报》 2003年第8期

膀胱肿瘤,,膀胱肿瘤；尿脱落细胞；微卫星改变；6号染体长臂,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (陕西医学高等专科学校生物化学教研室，陕西西安 710068，西安交通大学：医学院微生物学教研室，第一附属医院泌尿外科，陕西西安 710061)

    Investigation on microsatellite alteration at chromosome 6q of urine sediment in bladder tumor

    CHENG Fan, CHU YongLie, HE DaLin, YANG Lin, CHEN Ping, YANG E

    Department of Biochemistry,Shaanxi Medical College, Xian 710068,China, Department of Microbiology，Medical College,Department of Urology, First Affiliated Hospital of Xian Jiaotong University, Xian 710061, China

    【Abstract】AIM： To investigate the microsatellite alteration (MA) at chromosome 6q in the early diagnosis of bladder tumor and to study whether the loss of heterozygosity (LOH) at 6q is related to the development of bladder tumor. METHODS： D6S404 and D6434 microsatellite markers near 6q21 were tested by PCRSSLPstain method in both the urine sediment DNA and tumor DNA from 31 cases of bladder tumor. RESULTS： Among the 31 cases of bladder tumor, 64.5% of the tumor tissue DNA and 581% of the urine sediments DNA showed MA, while the 10 nonbladdertumor cases had no changes at either of the two loci. The result was not associated with the grade or stage of the tumor. LOH was detected in tumor tissues on site for D6S404 (355%) and D6S434 (226%). CONCLUSION： Microsatellite analysis of urine sediments is of significance in the early diagnosis of bladder tumor and there may be some tumor suppressor gene relevant to the development of bladder tumor near 6q21. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/02/19/87/26/34.htm

您现在查看是摘要页，全文长 7829 字符。