免疫血清_____人lrg基因；亲和纯化；免疫血清

人lrg在大肠杆菌中的表达及表达产物的免疫血清制备和鉴定

http://www.100md.com 《第四军医大学学报》 2004年第16期

免疫血清,,人lrg基因；亲和纯化；免疫血清,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Expression of human lrg in E.coli and identification of immune serum against human Lrg protein

    DU KeJun, CHAI YuBo, CHANG WenHui, HOU LiChao, ZHANG XiaoNan, CHEN NanChun,LIN ShuXin, CHEN SuMin

    Department of Biochemistry & Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To express human lrg in E.coli and to obtain an anti-Lrg immune serum. METHODS: lrg cDNA was cloned into pcTAT vector. The fusion proteins were expressed in E.coli and purified by Ni-NTA column and SDS-PAGE. Rabbits were immunized with preliminary purified Lrg protein and reinforced three times. The antiserum was collected. RESULTS: Antiserum against Lrg was obtained in immunized rabbits and its titer was more than 1∶1000 proven by ELISA and Western Blot. The purified Lrg protein could get across membranes of Papilla Dental Cells within 30 min. Immunohistochemical test with this antiserum showed that Lrg was a cytoplasmic protein and rabbit antiserum against human Lrg protein could be used in eukaryotic cell and normal tissues. CONCLUSION: The immune serum specific against Lrg is obtained, which can be used in normal tissues and cells. ......

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