骨髓基质细胞_____骨髓基质细胞；膀胱；

新西兰兔骨髓基质细胞构建组织工程膀胱的实验研究

http://www.100md.com 《第四军医大学学报》 2003年第8期

骨髓基质细胞,,骨髓基质细胞；膀胱；,移植；,组织工程,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学: 西京医院泌尿外科，实验动物中心，陕西西安 710033，解放军148医院泌尿外科，山东淄博 255300)

    Marrow stromal cells application: Reconstruction of the bladder of New zealand white rabbits by tissue engineering techniques

    YAN Quanjian, LI LiuJin, ZHANG YuMei, Yang GuiZhong, LIU HaiJun, GUO JinLong, WANG He, SHAO GuoXing

    Department of Urology, Xijing Hospital,Center of Laboratory Animal, Fourth Military Medical University, Xian 710033, China,Department of Urology, 148th Hospital, Zibo 255300, Shandong，China

    【Abstract】 AIM: To investigate the function parameters of tissue engineered bladders with marrow stromal cells(MSCs). METHODS： The MSCs and the bladder-shaped accellular organ specific matrix (BAMG) were obtained from the New Zealand White rabbits. The exterior surface of BAMG was seeded with the resuspended MSCs. A total of 20 rabbits underwent a trigone-sparing cystectomy. The animals were randomly assigned to three groups. Group A (n=6) underwent a closure of the trigone without a reconstructive procedure. Group B (n=7) underwent a reconstruction using a BAMG only and Group C (n=7) underwent a reconstruction using a BAMG plus autologous MSCs. Preoperative and postoperative cystometry and radiography studies were performed serially. Animals were euthanized at 12 weeks. Gross and histological analyses were also performed. RESULTS： The average time elapsed between the initial marrow biopsy and the final implantation of the tissue-engineeredneobladders was (21±3) days (x±s). Post operation, the average capacities in Group A, B and C were 22%, 85% and 95%, respectively and showed a marked difference in bladder compliance (11%, 104%, and 107% respectively). Histologically, group C showed a normal cellular organization consisting of a trilayer of urothelium, submucosa and muscle. The Group A, B presented a pattern of normal urothelial cells with a thickened fiberotic submucosa and a thin layer of muscle fibers. CONCLUSION： Our study demonstrates that the MSCs can be successfully used in rabbits bladder augmentations and replacement by tissue engineering techniques. ......

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