肾移植_____霉酚酸；肾移植；药代动力学；高效液相色谱法

HPLC法测定肾脏移植患者血浆中霉酚酸浓度及其药代动力学

http://www.100md.com 《第四军医大学学报》 2004年第15期

肾移植,,霉酚酸；肾移植；药代动力学；高效液相色谱法,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Determination of mycophenolic acid in plasma of kidney transplantation patients by HPLC and its pharmacokinetic

    SHI Ru, WEN AiDong, ZHAO Lei, LIN Lin, WANG Meng, WU Yin

    Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, China

    【Abstract】 AIM: To determine mycophenolic acid (MPA) in plasma of kidney transplantation patients by HPLC and its pharmacokinetics. METHODS: Discover C18(250.0 mm×4.6 mm, 5 μm)spectrum analytical column was used and phosphate bufferacetonitrile(45∶55) was adopted as the mobile phase. Flow rate was 0.8 mL/min, detection wavelength was 250 nm, column temperature was 30℃ and the dosage of MPA was 100 μL. The pharmacokinetic parameters of MPA taken by 9 kidney transplantation patients were standard curve calculated in plasma. RESULTS: The standard curve was linear (r=0.9997) with the range of 0.0995-27.8516 mg/L for MPA. The limit of detection in plasma was 5 μg/L. The average recovery were 96.30%, 97.82% and 98.62%, respectively. The interday precision and intraday precision were 3.6%，1.8%, 0.5% and 5.6%, 3.2%, 1.3%, respectively. The results showed that the pharmacokinetic of MPA was in accordance with the onecompartment open model. Tmax, Cmax and AUC(0-12 h) was 0.93 h ......

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