Mouse marrow stromal cell differentiates into cardiomyocyte progenitor

    ZHANG Yong, CAI Zhenª²Jie, CHEN Ruª²Kun

    1Department of Thoracic and Cardiovascular Surgery, Second Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310009, China, 2Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the differentiation from mouse mesenchymal stem cell (MSCs) into cardiomyocyte progenitor. METHODS: MSCs were separated from mouse marrow and cultured and observed. To detect the surface antigens, the label cells were analyzed on a FAC scan flow cytometer. MSCs were induced with 5ª²azacytidine which acted as a myoblast inducer. The induced MSCs were detected by RTª²PCR, electron microscope and immunofluorescence technique. RESULTS: The isolated subculturing MSCs displayed a fusiform cellª²like morphology. MSCs were uniformly positive for CD29 and CD44, but didn¡¯t express CD34 and CD45. Through RTª²PCR analysis, induced MSCs expressed cardiomyocyteª²specific transcription factors (NKx2ª²5/Csx and GATA4) and fetal ventricular cardiomyocyteª²specific gene ¦Âª²myosin heavy chain (¦Âª²MHC), but did not express adult ventricular cardiomyocyteª²specific geneª²myosin heavy chain (¦Áª²MHC). Induced MSCs expressed ¦Áª²sarcomeric actin and desmin by immunofluorescence technique and myofilament formation was seen under electron microscope. CONCLUSION: MSCs can be induced to differentiate into cardiomyocyte progenitor. ......