钙离子_____钙离子；类缺血；丹曲林钠

丹曲林钠对类缺血小鼠皮质神经元的[Ca2+]i和细胞活力的影响

http://www.100md.com 《第四军医大学学报》 2003年第7期

钙离子,,钙离子；类缺血；丹曲林钠,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学西京医院神经内科，陕西西安 710033)

    Effects of dantrolene on intracellular [Ca2+] and cell activity in cultured cortical neurons following ischemiclike conditioning

    GAO JunXian, WAN Qi, TIAN Ye, LI Li, LIU YongHong

    Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study the changes of intracellular [Ca2+] and cell activity in cortical neurons following ischemiclike conditioning and to explore the protective effects of dantrolene on the neurons. METHODS: Neurons were cultured and loaded with Flu3/AM, [Ca2+]i was measured by fluorescent intensity (FI) in each cell with confocal microscopy and the degree of injured neurons was analyzed with MTT reduction assay. RESULTS: After 10 min ischemiclike conditioning, a rapid increase in fluorescence intensity was observed. There was no difference between ischemic group and therapy group (P>0.05). The A value of injured neurons in ischemic group was significantly lower than that in therapy group. CONCLUSION: Dantrolene inhibits the increase of [Ca2+]i in cortical neurons and alleviates the degree of the injured neurons following ischemiclike conditioning. ......

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