大鼠溃疡性结肠炎模型肠组织中MIF的表达和NF-κB的激活
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黄 超, 邓长生
溃疡性结肠炎;巨噬细胞移动抑制因子;核因子-κB,黄超,邓长生,通讯作者:,Expressionofmacrophagemigration
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黄超, 邓长生, 武汉大学中南医院消化内科 湖北省武汉市 430071
通讯作者: 黄超, 430071, 湖北省武汉大学中南医院消化内科. hc2558@126.com
电话: 027-87330294 传真: 027-87330294
收稿日期: 2005-12-12 接受日期: 2005-12-31
Expression of macrophage migration inhibitory factor and activation of nuclear factor-κB in colon tissues of rats with ulcerative colitis
Chao Huang, Chang-Sheng Deng
Chao Huang, Chang-Sheng Deng, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China
Correspondence to: Chao Huang, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China. hc2558@126.com
Received: 2005-11-23 Accepted: 2005-11-25
Abstract
AIM: To detect the expression of macrophage migration inhibitory factor (MIF) and nuclear factor-κB (NF-κB) in the colon tissues of rats with ulcerative colitis, and investigate the role of MIF in pathogenesis of ulcerative colitis.
METHODS: Twenty-four Sprague-Dawley rats were randomly divided into three groups: normal group (n = 8), model groupⅠ(n = 8), model groupⅡ(n = 8); The rat in model groupⅠ and Ⅱ treated with 50 and 100 mg/kg 2,4,6-trinitrobenzene sulfonic acid (TNBS), respectively, to establish light and severe colitis model. The disease activity index (DAI) was calculated every day, and the expression of MIF in colon tissues was detected by semi-quantitative reverse transcription chain reaction (RT-PCR). The expression of NF-κB was examined by immunohistochemistry, and the myeloperoxidase (MPO) activity was detected by biochemistry.
RESULTS: In normal group, the MPO activity, DAI, NF-κB and MIF mRNA level were 0.38 ± 0.18 U/g, 0.51 ± 0.28, 0.18 ± 0.05 and 0.11 ± 0.03, respectively; in model groupⅠthey were 1.68 ± 0.45 U/g, 5.04 ± 0.73, 0.62 ± 0.08 and 0.65 ± 0.04, respectively; in model groupⅡthey were 2.70 ± 0.35 U/g, 8.13 ± 0 ......
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